P-56: Artificial Oocyte Activation Alone Intra Cytoplasmic Sperm Injection in Men with Severe Semen Parameters

Authors

  • M Bahadorani Department of Biology, Islamic Azad University, Falavarjan University, Isfahan, Iran
  • M Norozi Department of Biology, Islamic Azad University, Falavarjan University, Isfahan, Iran
  • M Tavalaee Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
  • MH Nasr Esfahani Department of Reproductive Biotechnology, Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran
Abstract:

Background Failed fertilization occurs 1-3% in the infertile couples. Failure of oocyte activation (OA) is mainly attributed to failed fertilization. ICSI alone artificial oocyte activation (AOA) is only treatment approach for these individuals. Three procedures are introduced for AOA: chemical, mechanical and electrical. Most of the clinical Centers have commonly used ionomycin for activation of oocyte in the human. In this regard, it is reported that ionomycin could be a suitable agent for infertile couples’ candidate of ICSI. Therefore, this clinical study was designed to compare clinical outcomes of infertile men who oocytes of their wife were treated with ionomycin and who oocytes of their wife were not treated with ionomycin (control). MaterialsAndMethods Semen samples were obtained from 30 infertile individuals with severe male factor. 15 couples candidate of AOA were considered as “study group” and treated with 10 M ionomycin. 15 couples were not treated with ionomycin and considered as “control group”. Fertilization rate, embryo quality and pregnancy rate were compared between two groups. Results Percentage of fertilization rate and cleavage rate were significantly higher in study group compared to control group (P0.05). Conclusion We suggested that AOA along with ICSI may improve the chance of fertilization and cleavage rate in couples with severe abnormal semen.

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Journal title

volume 9  issue 2

pages  67- 67

publication date 2015-09-01

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